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In addition, the brink volume of CYC9 or CRK12 necessary for every of those capabilities could possibly be distinct, so that depletion of CYC9 or CRK12 can be ample to disrupt cytokinesis or endocytosis, although not each.To investigate the subcellular localization on the CRK12 protein, a pEarleyGate104 vector was used for a transient expression wi